1，天然产物生物合成
挖掘参与活性天然产物生物合成相关的途径酶基因，并对其进行功能表征；通过计算机模拟以及定向进化等手段对关键途径酶进行突变，获得活性显著提升，具有预设催化特性的生物催化剂，用于天然产物的酶法合成。
2，合成生物学技术制备天然产物
在不同底盘细胞中重构天然产物生物合成代谢途径及调控网络，获得能制备天然产物的细胞工厂，用于天然产物的生物合成。

1. Shu-Yue Zhang, Jian-Qiang Kong*, The clarification of two hydroxylation steps in the conversion of cholesterol to OSW-1 in Ornithogalum saundersiae, Plant Biotechnology Journal, 2025, DOI: 10.1111/pbi.70080
2. Xue-Ning Wang, Li Sheng, Xin-Yi Guo, Hua Sun, Jian-Qiang Kong*, Green acetyl modification of puerarin to form puerarin 6″-O-acetate using engineered Escherichia coli with favorable pathways and elevated acetyl-CoA supply, Green Chemistry, 2024, 26(6):3249-3260.
3. Li-li Hong, Yuan-Ning Liu, Jian-Qiang Kong*, Exploring 3-O-glycosylations of 20(R)-dammarane ginsenosides and the catalytic mechanism underlying the stereoselectivity with the combined assistance of AlphaFold 2 and molecular docking, International Journal of Biological Macromolecules, 2024,254:127721.
4. Zhen Xu, Li-li Hong, Chun-Sheng Liu*, Jian-Qiang Kong*, Protein engineering of PhUGT, a donor promiscuous glycosyltransferase, for the improved enzymatic synthesis of antioxidant quercetin 3-O-N-acetylgalactosamine. Journal of Agricultural and Food Chemistry, 2022, 70(13): 4076-4085.
5. Yuan-Ning Liu, Li-Li Hong, Ming Liu, Qing-Chun Guo, and Jian-Qiang Kong*, Glycodiversifying testosterone with a promiscuous glycosyltransferase OsSGT2 from Ornithogalum saundersiae, ACS Synthetic Biology, 2021, 10(12): 3583-3594.
6. Xue-Ning Wang, Li-Li Hong, and Jian-Qiang Kong*, Diacerein as a promising acyl donor in biosynthetic acetyl-coa and glycosyl esters mediated by a multifunctional maltose O‑acetyltransferase from Escherichia coli, Journal of Agricultural and Food Chemistry, 2021, 69(23): 6623-6635.